Mika Takahashi1, Hina Kato1, Yuko Doi2, Akihiro Hagiwara2, Mutsuko Hirata-Koizumi1, Atsushi Ono1, Reiji Kubota3, Tetsuji Nishimura3 and Akihiko Hirose1
To obtain initial information on the possible repeated-dose oral toxicity of fullerene C60, Crl:CD(SD) rats were administered fullerene C60 by gavage once daily at 0 (vehicle: corn oil), 1, 10, 100, or 1,000 mg/kg/day for 29 days, followed by a 14-day recovery period. No deaths occurred in any groups, and there were no changes from controls in detailed clinical observations, body weights, and food consumption in any treatment groups. Moreover, no treatment-related histopathological changes were found in any organs examined at the end of the administration period and at the end of the recovery period. Blackish feces and black contents of the stomach and large intestine were observed in males and females at 1,000 mg/kg/day in the treatment group. There were no changes from controls in the liver and spleen weights at the end of the administration period, but those weights in males in the 1,000 mg/kg/day group increased at the end of the recovery period. Using liquid chromatography-tandem mass spectrometry, fullerene C60 were not detected in the liver, spleen or kidney at the end of the administration period and also at the end of the recovery period. In conclusion, the present study revealed no toxicological effects of fullerene C60; however, the slight increases in liver and spleen weights after the 14-day recovery period may be because of the influence of fullerene C60 oral administration.
Update on C60 fullerenes in olive oil | AgingSciences - Anti-Aging Firewalls™
By Vince Giuliano and James P Watson
More than a year has passed since publication of the November 2012 blog entry Buckyballs, health and longevity – state of knowledge, It will be another year or two before we know whether researchers are capable of reproducing the extraordinary longevity impacts on rats reported in the 2011 publication The prolongation of the lifespan of rats by repeated oral administration of  fullerene.
BioMed Research International
Novel mechanism of antioxidant activity of buckminsterfullerene C60 based on protons absorbing and mild uncoupling of mitochondrial respiration and phosphorylation was postulated. In the present study we confirm this hypothesis using computer modeling based on Density Functional Theory. Fullerene's geroprotective activity is sufficiently higher than those of the most powerful reactive oxygen species scavengers. We propose here that C60 has an ability to acquire positive charge by absorbing inside several protons and this complex could penetrate into mitochondria. Such a process allows for mild uncoupling of respiration and phosphorylation. This, in turn, leads to the decrease in ROS production.
SciVerse ScienceDirect and Elsevier.com
Countless studies showed that fullerene (C(60)) and derivatives could have many potential biomedical applications. However, while several independent research groups showed that C(60) has no acute or sub-acute toxicity in various experimental models, more than 25 years after its discovery the in vivo fate and the chronic effects of this fullerene remain unknown. If the potential of C(60) and derivatives in the biomedical field have to be fulfilled these issues must be addressed. Here we show that oral administration of C(60) dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. The effects of C(60)-olive oil solutions in an experimental model of CCl(4) intoxication in rat strongly suggest that the effect on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Pharmacokinetic studies show that dissolved C(60) is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours. These results of importance in the fields of medicine and toxicology should open the way for the many possible -and waited for- biomedical applications of C(60) including cancer therapy, neurodegenerative disorders, and ageing.
The prolongation of the lifespan of rats by repeated oral administration of fullerene (PDF Download Available). Available from: https://www.researchgate.net/publication/224004891_The_prolongation_of_the_lifespan_of_rats_by_repeated_oral_administration_of_60fullerene [accessed Sep 1, 2017].
By Marsha McCulloch, RD
Olive oil has been pinpointed as a major source of the cardiovascular benefits long associated with a Mediterranean diet.1,2 A recent study of a healthy Mediterranean population showed that olive oil consumption accounted for as much as a 44% reduction in cardiovascular deaths compared to those who didn’t use olive oil.3
Kevin L. Quick a, Sameh S. Ali a, Robert Arch b, Chengjie Xiong c, David Wozniak d, Laura L. Dugan a,∗
In lower organisms, such as Caenorhabditis elegans and Drosophila, many genes identified as key regulators of aging are involved in either detoxification of reactive oxygen species or the cellular response to oxidatively-damaged macromolecules. Transgenic mice have been generated to study these genes in mammalian aging, but have not in general exhibited the expected lifespan extension or beneficial behavioral effects, possibly reflecting compensatory changes during development. We administered a small-molecule synthetic enzyme superoxide dismutase (SOD) mimetic to wild-type (i.e. non-transgenic, non-senescence accelerated) mice starting at middle age. Chronic treatment not only reduced age-associated oxidative stress and mitochondrial radical production, but significantly extended lifespan. Treated mice also exhibited improved performance on the Morris water maze learning and memory task. This is to our knowledge the first demonstration that an administered antioxidant with mitochondrial activity and nervous system penetration not only increases lifespan, but rescues age-related cognitive impairment in mammals. SOD mimetics with such characteristics may provide unique complements to genetic strategies to study the contribution of oxidative processes to nervous system aging. © 2006 Elsevier Inc. All rights reserved.
Alexander V. Syrensky,1 Elena I. Egorova,2 Ilia V. Alexandrov,2 and Michael M. Galagudza2, 1 V.A. Almazov Research Institute of Cardiology of the Ministry of Health Care and 2 St. Petersburg I.P. Pavlov Federal Medical University, St. Petersburg, Russian Federation
During two last decades, several unique physical and chemical properties of buckminsterfullerene or fullerene C60 have been described. However, much less is known about the effects of fullerenes and their derivatives on biological systems. Evidence is beginning to accumulate that fullerenes may exert influence on different physiological and pathophysiological processes primarily because of their antioxidant effects.
Sean Rossman, USA TODAY NETWORK, June 21, 2017
Temple University research shows extra-virgin olive oil protects against memory loss, preserves the ability to learn and reduces conditions associated with Alzheimer's disease
Researchers at the college's Lewis Katz School of Medicine found mice with EVOO-enriched diets had better memories and learning abilities compared to the rodents who didn't eat the oil.
The real effect of EVOO appeared in the inner-workings of the mice's brains. Neuron connections in the brain were better preserved in those on an EVOO diet.
S.V. Prylutska,*, A.P. Burlaka , Yu.I. Prylutskyy , U. Ritter , P. Scharff
Aim: To estimate the impact of C60 fullerene aqueous solution (C60FAS) on the rate of transplanted malignant tumor growth and metastasis. Methods: Lewis lung carcinoma was transplanted into С57Bl/6J male mice. Conventional methods for the evaluation of antitumor and antimetastatic effects have been used. Results: The C60FAS at low single therapeutic dose of 5 mg/kg inhibited the growth of transplanted malignant tumor (antitumor effect) and metastasis (antimetastatic effect): the maximum therapeutic effect was found to be of 76.5% for the tumor growth inhibition; the increase of animal life span by 22% was found; the metastasis inhibition index was estimated as 48%. Conclusion: It was found that water-soluble pristine С60 fullerenes efficiently inhibit the transplanted malignant tumor growth and metastasis.
Oxidative Stress in Chronic Obstructive Pulmonary Disease
Chronic obstructive pulmonary disease (COPD) is a major worldwide health problem that has an increasing prevalence and mortality (1, 2). Oxidative stress, which can be defined as an increased exposure to oxidants and/or decreased antioxidant capacities, is widely recognized as a central feature of many diseases (3, 4). Considerable evidence now links COPD with increased oxidative stress (5, 6). The purpose of this review is to describe the role and origin of the oxidant–antioxidantdisturbances that participate in the development of COPD. Our presentation also addresses ways of assessing the contribution of oxidants and identifies therapeutic approaches that could improve cellular oxidant–antioxidant balance in the lungs of COPD patients.